Alliance HPLC System

Chromatography Systems

Choose the right LC for your analysis.

It’s your laboratory – so why shouldn’t you choose the system that’s right for you?

Whether you’re looking to transfer methods from your existing HPLC or UHPLC system, running routine analysis, or looking for a powerful inlet to MS, Waters puts you in the driver’s seat with our portfolio of LC systems.

Category: Tags: , Brand: Waters

Description

Defined by dependability – today, and in the future

  • Time-tested, flexible, and reliable workhorse for your HPLC separations
  • Integrated solvent and sample management with consistent system-to-system performance and high reproducibility
  • A history of continued system improvements ensures the hightest in HPLC performance
  • Simplify your daily LC system start-up with advanced software functionality

Excellence through the years

The consistency of the Alliance HPLC System gives you exceptional confidence in your analytical results, day-to-day and year-to-year.

Get accurate and reproducible results with Waters’ flagship HPLC technology, which provides consistent and reliable performance whether your Alliance HPLC System is newly manufactured, or you’re working on one that is several years old.

Comparison of the separation of carbamates using two different Alliance HPLC Systems, manufactured 7 years apart. The results are equivalent.

Performance that exceeds your method requirements

High injection precision for precise quantification, and exceptional retention time repeatability aid in accurate peak identification, the Alliance HPLC System ensures the reliability of your results.

The solvent delivery system features quaternary blending with a fixed delay volume for consistent and predictable results, regardless of system back pressure. Flexible injection capabilities cover a wide injection volume range (up to 2 mL) with excellent carryover performance, typically below 0.005%.

zoom

USP method for Budesonide on the Alliance HPLC System.

zoom

By adding mass detection with the ACQUITY QDa Detector to an existing Alliance HPLC System with UV detection, the peaks of interest in this Golden Seal sample can be easily identified by the m/z values.

Advancements in LC chemistry and mass detection

Improve your laboratory’s efficiency using CORTECS 2.7 μm Columns with your Alliance HPLC System. Benefit from improvements in both resolution and a reduction in run times when transferring from a 5-μm chemistry.

Increase confidence in your data by adding mass confirmation with the ACQUITY QDa Detector, which is as simple to use as a photodiode array detector and can easily integrate into your existing workflow. Analysis in Empower 3 Software combines the data from the UV/Vis and MS peaks to give mass information to each peak in the separation.

Features and Benefits

  • Quaternary solvent blending supports isocratic and gradient applications with 11 unique gradient profile curves. Blend up to 4 solvents in any proportion with Auto-Blend Technology. Compatible with a wide range of solvent types to support reversed-phase, normal-phase, GPC, SEC, and IEX application.
  • Advanced solvent delivery capabilities. Serial flow path with pulse-free solvent delivery without the use of dampeners. Online solvent degassing and automated solvent compressibility for accurate flow. Integrated seal wash for robustness operation with all solvent types is standard.
  • Up to 120 vials housed in an optional, temperature-controlled environment.  Active and user programmable needle wash for low carryover.  Variable injection volumes from 0.1 to 100 uL standard, and up to 2 mL optional.  Supports STAT samples and auto-addition.
  • Optional column heating or heating/cooling. Get the flexibility of running multiple methods on demand with optional column switching.
  • Detection capabilities include photodiode array, UV/Vis, fluorescence, refractive index, evaporative light scattering, electrochemical, conductivity, and mass detection.
  • An intuitive user interface, giving you instant access to system settings through the large LCD screen. Simplified daily set-up with SystemPREP.
  • Supported in Empower, MassLynx, and several 3rd party software packages
  • Compliance-ready. Qualification of laboratory instrumentation is an integral part of compliance with regulatory and quality standards such as GMP, GLP, ISO 17025, USP <1058>, and others.  The Alliance HPLC System supports Waters Empower-based automated system qualification tool (SQT), which minimizes the time and cost associated with annual qualification.
Description Year
Alliance HPLC System
Format:  PDF   |   File Size:  3805.69 kB   |   Content Subtype:  Brochures
2018
Alliance HPLC Systems for Carbamate Analysis Brochure
Format:  PDF   |   File Size:  916.71 kB   |   Content Subtype:  Brochures
2004
Waters Corporate Capabilities Brochure
Format:  PDF   |   File Size:  1771.76 kB   |   Content Subtype:  Brochures
2015
Waters Total Assurance Warranty for Waters Systems
Format:  PDF   |   File Size:  1492.89 kB   |   Content Subtype:  Brochures
2014
Alliance HPLC System Accessories
Format:  PDF   |   File Size:  1450.73 kB   |   Content Subtype:  Brochures
2013
Achievement in Service Excellence – Making Our Customers Successful
Format:  PDF   |   File Size:  1964.94 kB   |   Content Subtype:  Brochures
2016
XSelect HPLC Columns
Format:  PDF   |   File Size:  2822.47 kB   |   Content Subtype:  Brochures
2015
Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer
Format:  PDF   |   File Size:  1007.76 kB   |   Content Subtype:  White Papers
2018
LC Portfolio Brochure
Format:  PDF   |   File Size:  2574.97 kB   |   Content Subtype:  Brochures
2018
e2695 Separations Module Instrument Specification
Format:  PDF   |   File Size:  256.7 kB   |   Content Subtype:  Specifications
2018
Waters Natural Products Solutions: Helping You Overcome The Complexities Of Natural Products
Format:  PDF   |   File Size:  2329.98 kB   |   Content Subtype:  Brochures
2016
Transfer of an HPLC Method from an Agilent 1100 LC System to an Alliance HPLC System
Format:  PDF   |   File Size:  116.99 kB   |   Content Subtype:  Brochures
2015
Developing Liquid Chromatography Methods for Food Analysis
Format:  PDF   |   File Size:  2234.88 kB   |   Content Subtype:  Brochures
2013
Description Year
Determination of Food Sugars in Fruit Juice Using Evaporative Light Scattering Detection and BEH Amide Column Chemistry
Format:  PDF |   File Size:  1126.01 kB –
2013
Determination of Food Sugars in Fruit Juice Using Refractive Index Detection and BEH Amide Column Chemistry
Format:  PDF |   File Size:  1054.26 kB –
2013
Evaluation of the Alliance HPLC System to Separate Budesonide Epimers following the USP Compendial Method
Format:  PDF |   File Size:  1133.51 kB –
2013
Reliable and Robust Sample Injection with the Alliance HPLC System
Format:  PDF |   File Size:  1530.1 kB –
2013
Determination of Food Sugars in Fruit Juice Using Evaporative Light Scattering Detection and BEH Amide Column Chemistry
Format:  PDF |   File Size:  1126.01 kB –
2013
High Performance USP Analysis of Ziprasidone Capsules on an Alliance HPLC System
Format:  PDF |   File Size:  805.6 kB –
2013
Performance Verification of the USP Mometasone Furoate Ointment Method Using the Alliance HPLC System
Format:  PDF |   File Size:  886.22 kB –
2012
Analysis of Vegetable Oils by High Performance Liquid Chromatography Using Evaporative Light Scattering Detection and Normal Phase Eluents
Format:  PDF |   File Size:  934.25 kB –
2008
Evaluating the Impact of LC System Dispersion on the Size-Exclusion Chromatography Analysis of Proteins
Format:  PDF |   File Size:  815.2 kB –
2019
Gradient Method Scaling for Life Cycle Management of a USP Impurities Method
Format:  PDF |   File Size:  745.64 kB –
2019
Scaling USP Methods to Smaller Particle Columns on the Alliance HPLC System
Format:  PDF |   File Size:  402.07 kB –
2019
Accurate Bioanalytical Peptide Quantification of Salmon Calcitonin Using High Resolution Mass Spectrometry (HRMS)
Format:  PDF |   File Size:  413.15 kB –
2018
Alliance Carryover Performance Part 1: Carryover Improvement Achieved Through Instrument Design Changes for the Alliance HPLC System
Format:  PDF |   File Size:  343.51 kB –
2018
Alliance Carryover Performance Part 2: Carryover Improvement Achieved Through Needle Wash Optimization for the 2018 Alliance HPLC System
Format:  PDF |   File Size:  198.43 kB –
2018
Demonstration of the USP Quetiapine Fumarate Impurities Method across a Wide Range of Liquid Chromatographic Systems
Format:  PDF |   File Size:  309.91 kB –
2018
Evaluating HPLC, UHPLC, and UPLC System Performance Using a Reversed-Phase Method for Peptides
Format:  PDF |   File Size:  446.29 kB –
2018
Protein A for Monoclonal Antibody Titer Measurements with ACQUITY Arc Bio, Alliance, and ACQUITY UPLC H-Class Bio Chromatography Systems
Format:  PDF |   File Size:  629.95 kB –
2018
Scaling of a USP Assay for Quetiapine Fumarate Across Different Liquid Chromatographic Systems
Format:  PDF |   File Size:  1518.97 kB –
2018
Separation of 16 Cannabinoids in Cannabis Flower and Extracts Using a Reversed Phase Isocratic HPLC Method
Format:  PDF |   File Size:  403.53 kB –
2018
Transfer of USP Assay for Quetiapine Fumarate Across Different Liquid Chromatographic Systems
Format:  PDF |   File Size:  258.34 kB –
2018
HPLC and UHPLC Application Highlights Notebook
Format:  PDF |   File Size:  15485.77 kB –
2017
Improved Performance of Waters Alliance HPLC System for System Suitability Results of Area RSD for Eight Challenging Pharmacopoeial and Non-Pharmacopoeial Assay Methods
Format:  PDF |   File Size:  132.1 kB –
2017
USP Method Modernization Using “Equivalent L/dp” and “Equivalent N” Allowed Changes with CORTECS C8 and CORTECS UPLC C8 Columns
Format:  PDF |   File Size:  1127.08 kB –
2016
Characterizing of the Natural Product Goldenseal using Cortecs 2.7 μm Columns and ACQUITY QDa Detection
Format:  PDF |   File Size:  1720.1 kB –
2015
Peptide Isolation Using the Prep 150 LC System
Format:  PDF |   File Size:  1872.95 kB –
2015
Description Year
Prep 150 LC System: Considerations for Analytical to Preparative Scaling
Format:  PDF |   File Size:  1886.44 kB –
2015
Successful Transfer of Size-Exclusion Separations between HPLC and UPLC
Format:  PDF |   File Size:  1697.49 kB –
2015
Transfer of an HPLC Method from an Agilent 1100 Series LC System to an Alliance HPLC System
Format:  PDF |   File Size:  927.96 kB –
2015
Ultimate Resolution HPLC-Based Size Exclusion Chromatography for the Analysis of Small Proteins and Peptides Using 3.5 μm Ethylene Bridged Hybrid (BEH) Particles
Format:  PDF |   File Size:  1595.62 kB –
2015
Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 μm Ethylene Bridged Hybrid (BEH) Particles
Format:  PDF |   File Size:  1814.57 kB –
2014
Analysis of 2- and 4-Methylimidazole in Beverages using Alliance HPLC with Mass Detection
Format:  PDF |   File Size:  1773.03 kB –
2014
Detection of UV-transparent Compounds by Addition of a Mass Detector to an Existing High Performance Liquid Chromatography System with Photodiode Array Detection
Format:  PDF |   File Size:  894.76 kB –
2014
Forced Degradation Analysis of Omeprazole Using CORTECS 2.7 μm Columns
Format:  PDF |   File Size:  1440.18 kB –
2014
Repeatability and Quantitation of a Method Using the Alliance HPLC System with the 2998 PDA Detector and the ACQUITY QDa Detector
Format:  PDF |   File Size:  1548.69 kB –
2014
Transferring HPLC Gradient Methods Using CORTECS Solid-Core Particle Columns
Format:  PDF |   File Size:  1516.9 kB –
2014
Transferring the USP Assay of Zidovudine Using CORTECS 2.7 μm Columns
Format:  PDF |   File Size:  1635.06 kB –
2014
Comparing the Performance and Reliability of Waters Alliance HPLC Systems for Carbamate Analysis
Format:  PDF |   File Size:  1594.6 kB –
2013
Determination of Food Sugars in Fruit Juice Using Refractive Index Detection and BEH Amide Column Chemistry
Format:  PDF |   File Size:  1054.26 kB –
2013
Evaluation of the Alliance HPLC System to Separate Budesonide Epimers following the USP Compendial Method
Format:  PDF |   File Size:  1133.51 kB –
2013
High Performance USP Analysis of Ziprasidone Capsules on an Alliance HPLC System
Format:  PDF |   File Size:  805.6 kB –
2013
Improving Performance and Throughput of the USP Organic Impurities Analysis of Tioconazole on an Alliance HPLC System
Format:  PDF |   File Size:  1764.74 kB –
2013
Low Sample Carryover with Key Performance Indicators on the Alliance HPLC System
Format:  PDF |   File Size:  881.6 kB –
2013
Low Sample Carryover with Key Performance Indicators on the Alliance HPLC System
Format:  PDF |   File Size:  881.6 kB –
2013
Performance Verification of the USP Mometasone Furoate Ointment Method Using the Alliance HPLC System
Format:  PDF |   File Size:  886.22 kB –
2013
Reliable and Robust Sample Injection with the Alliance HPLC System
Format:  PDF |   File Size:  1530.1 kB –
2013
Streamlining the Analysis of Oral Contraceptives Using the ACQUITY UPLC H-Class System
Format:  PDF |   File Size:  1723.09 kB –
2013
Transfer of Two USP Compendial Methods for Impurities of Ziprasidone HCl to a Single UPLC Method
Format:  PDF |   File Size:  2071.21 kB –
2013
Transfer of USP Methods for Impurities Analysis of Ziprasidone HCl between HPLC Systems and to UPLC
Format:  PDF |   File Size:  1588.33 kB –
2013
USP Method Transfer and Routine Use Analysis of Budesonide Nasal Spray from HPLC to UPLC
Format:  PDF |   File Size:  2072.17 kB –
2013
USP Method Transfer and Routine Use Analysis of Irbesartan Tablets from HPLC to UPLC
Format:  PDF |   File Size:  2207.91 kB –
2013
Description Year
USP Method Transfer and UPLC Method for Analysis of Mometasone Furoate Ointment
Format:  PDF |   File Size:  2043.98 kB –
2013
USP Method Transfer and UPLC Method for Analysis of Telmisartan Tablets
Format:  PDF |   File Size:  1730.35 kB –
2013
USP Method Transfer of Amoxicillin Oral Suspension from HPLC to UPLC
Format:  PDF |   File Size:  1330.72 kB –
2013
USP Method Transfer of Donepezil Tablets from HPLC to UPLC
Format:  PDF |   File Size:  2619.85 kB –
2013
USP Method Transfer of Lamotrigine from HPLC to UPLC
Format:  PDF |   File Size:  2066.25 kB –
2013
USP Method Transfer of Levonorgestrel and Ethinyl Estradiol Tablets from HPLC to UPLC
Format:  PDF |   File Size:  1860.92 kB –
2013
Modernization of the USP Assay for Clarithromycin Using eXtended Performance (XP) Column Technology
Format:  PDF |   File Size:  2372.94 kB –
2012
Transferring Compendial HPLC Methods to UPLC Technology
Format:  PDF |   File Size:  8116.54 kB –
2012
Performance and Verification of the USP Fluconazole Related Compounds Test on the Alliance HPLC System
Format:  PDF |   File Size:  1198.71 kB –
2009
Reducing Acetonitrile Usage for the HPLC Analysis of Aldehyde and Ketone Pollutants
Format:  PDF |   File Size:  1392.22 kB –
2009
Determination of Aflatoxins Utilizing XBridge HPLC Columns
Format:  PDF |   File Size:  1342.11 kB –
2008
Extending High Performance Liquid Chromatography Method Development Capabilities by Adding Single Quadrupole Mass Spectrometry Detection
Format:  PDF |   File Size:  2724.21 kB –
2008
High Throughput Analysis for Adenosine Injection USP
Format:  PDF |   File Size:  1168.24 kB –
2008
Analysis of Aminoglycoside Antibiotics with the Waters 2465 Electrochemical Detector
Format:  PDF |   File Size:  582.29 kB –
2004
Multi-Analyte Mycotoxin Analysis
Format:  PDF |   File Size:  295.38 kB –
2004
Waters Alliance System: Gradient Analysis of Polymer Blends
Format:  PDF |   File Size:  195.96 kB –
2001
Chromatographic System Requirements for Exploiting the Full Potential of Sub-2 µm Porous Particle LC
Format:  PDF |   File Size:  2243.35 kB –
2008
Description Date
ACQUITY Isocratic Solvent Manager (ISM) Firmware Version 1.72.647 Certificate of Structural Integrity May 1, 2019
e2695 Separations Module Version 3.04.798 Certificate of Structural Integrity April 3, 2018
Waters Driver Pack 2017 Release 2 Version 1.70 Certificate of Structural Integrity January 16, 2018
Alliance/Alliance HT SystemsQT Project/Documentation DVD Revision N Certificate of Structural Integrity November 27, 2017
Waters Driver Pack 2017 Release 1 Version 1.69 Certificate of Structural Integrity June 20, 2017
e2695 Separations Module Version 3.03.739 Certificate of Structural Integrity January 16, 2017
e2695 Separations Module Version 3.03 Certificate of Structural Integrity December 16, 2016
Alliance/Alliance HT SystemsQT Project/Documentation DVD Revision M Certificate of Structural Integrity November 27, 2017
ACQUITY Isocratic Solvent Manager (ISM) Firmware Version 1.65.268 Certificate of Structural Integrity July 13, 2015
Alliance/Alliance HT SystemsQT Project/Documentation DVD Revision L Certificate of Structural Integrity March 2, 2015
e2695 Separations Module (Blue) Firmware Revision 3.02.700 Certificate of Structural Integrity September 22, 2014
e2695 Separations Module (Platinum) Firmware Revision 3.02.700 Certificate of Structural Integrity September 22, 2014
e2695 Separations Module Firmware Revision 3.02 Certificate of Structural Integrity August 8, 2014
ACQUITY Isocratic Solvent Manager Certificate of Structural Integrity November 8, 2013
Alliance e2695 Separations Module (Blue) Firmware Version 3.00 Certificate of Structural Integrity February 22, 2013
Alliance e2695 Separations Module (Platinum) Firmware Version 3.00 Certificate of Structural Integrity August 4, 2008
2695 Separations Module Firmware Version 2.04 January 10, 2008
ConnectionsAQT for Waters Alliance Systems Version 1.20 February 28, 2008
ConnectionsAQT for Waters Alliance Systems Version 2.00 January 11, 2008
ConnectionsAQT for Waters Alliance Systems Version 2.20 January 11, 2008
ConnectionsAQT for Waters Alliance Systems Version 3.00 January 11, 2008
2695 Separations Module Firmware Version 3.00 August 4, 2008
Description Date
Alliance e2695 Separations Module Firmware Version 3.04 April 24, 2018
Waters Driver Pack 2017 Release 2 (DP 2017 R2) January 30, 2018
Alliance e2695 Separations Module Firmware Revision 3.03 January 16, 2018
Waters Driver Pack 2017 Release 1 (DP 2017 R1) June 22, 2017
e2695 Separations Module Firmware Revision 3.02 August 8, 2014
Alliance 2695 Separations Module Version 2.04 Firmware Update March 14, 2008

User Manuals

Description Date
2018 Alliance Enhancements – Quick Reference Guide June 28, 2018
RXN 1000 Reaction Coil Care and Use Instructions July 3, 2009
Alliance e2695 Separations Module Operator’s Guide March 6, 2018
Waters Driver Pack 2017 Release 2 Installation and Configuration Guide February 28, 2018
Waters Driver Pack 2017 Release 1 Installation and Configuration Guide November 17, 2017
Post-Column Reaction Module Installation Guide October 17, 2017
ACQUITY Isocratic Solvent Manager Overview and Maintenance Guide October 7, 2013
Major Contaminants and Their Sources July 9, 2013
Alliance System for Carbamate Analysis Method Guide May 20, 2013
Alliance Series Column Heater and Column Heater/Cooler Operator’s Guide February 22, 2013
Method Development: a Guide to Basics October 24, 2011
Guide to Successful Operation of Your LC System April 25, 2011
Column Bypass Module Installation and Maintenance Guide April 29, 2004
2695 Degasser Flowchart Reference Sheet May 4, 2004
2695 Separations Module Operator’s Guide May 4, 2004
2695 Separations Module Quick Start Guide May 4, 2004

Waters continued commitment to liquid chromatography reaches a new level with the latest Alliance HPLC System. Building on industry-standard performance and reliability around the world, this system combines the full capability to meet your business challenges with the flexibility that anticipates your future needs.

With its proven performance history, the Alliance HPLC is the industry’s analytical HPLC system of choice, ideally-suited for methods development and routine analysis. Swap out your current 5 μm column and enhance your laboratory’s analytical workflow, sample throughput, and chromatographic information with eXtended Performance [XP] 2.5 Columns. It’s that easy!

Title Format File Size
Download PDF PDF 3805.69kB